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KMID : 0613820040140050863
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Journal of Life Science
2004 Volume.14 No. 5 p.863 ~ p.867
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Antioxidative Effects of Pine (Pinus denstifora) Needle Extracts
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Yoo Ji-Hyun
Cha Jae-Young
Jeong Young-kee
Chung Kyung-Tae
Cho Young-Su
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Abstract
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Antioxidative activities of pine (Pinus denstifora) needle extracts were tested in vitro experimental models. The concentration of total polyphenolic compound of water extracts from pine needle was 1.61%. In DPPH (α,α‘-diphenyl-β-picrylhydrazyl) method, the electron donating activity of 0.1% water extracts from pine needle was as high as BHT (0.05%, w/v). The antioxidative activity was measured by inhibition against lipid peroxidation of rat liver microsome, and this activity was shown in the following : 67.7% at 0.1% concentration＞63.1% at 0.05% concentration＞28.2% at 0.01% concentration. In antioxidative activity determined by thiocyanate method against lipid peroxidation using linoleic acid, the antioxidative activities at all concentration of 0.01%, 0.05% and 0.1% were much higher than control during 7 days. In TBA method, the antioxidative activity was increased with increasing concentration until 6 days. These results support that water extracts from pine needle contain antioxidative compounds.
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KEYWORD
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Pind needle, polyphenolic compound, antioxidative activity, DPPH (α, α‘-diphenyl-β-picrylhydrazyl)
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